By Karl Maramorosch
Useful Tissue tradition Applications
summary: useful Tissue tradition purposes
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Additional resources for Practical Tissue Culture Applications
It is not unusual to find a difference of 2° or 3° between the temperature indicated and the actual temperature on the shelf where cell cultures are placed. D. Impurity of Water Fundamental to all tissue culture media and other solutions is the diluent water. In countries where tap water is soft and well purified, single distilled water or water passed through a deionizer can be used for cell cultures. However, in most countries where water is hard, tap water has to be distilled once or twice before it is further purified by either glass distillation or a deionizer.
1972). In " G r o w t h , N u t r i t i o n and M e t a b o l i s m of Cells in C u l t u r e " (G. H . Rothblat and V. J. Cristofalo, e d s . ) , Vol. 1, p p . 137-170. A c a d e m i c P r e s s , N e w Y o r k . Meistrich, M. L . , G a r d i n a , D. , M e y n , R. E . , and Barlogie, B. (1977). Cancer Res. 37, 4291-4296. , K a h n , R . H . , and M u r p h y , W . H . (1964). " H a n d b o o k of Cell and O r g a n C u l t u r e , " 2nd e d . B u r g e s s Publ. C o . , M i n n e a p o l i s , M i n n e s o t a .
Cloning has been practiced probably as long as man has cultivated plants, and tissue culture might be viewed as simply another method of clonal propagation. But it has the advantage over traditional methods of enabling substantially greater rates of plant multiplication. Many pathogens, especially bacteria and fungi, may be removed by the tissue culture process. The basic principles that apply to the propagation in vitro of any plant are established (Murashige, 1974b, 1977, 1979). Although some major problems remain, in most cases it is only necessary to apply the principles and to identify specific needs.
Practical Tissue Culture Applications by Karl Maramorosch